Coding

Part:BBa_J70630:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-08-21)


RBS-MBP-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 394
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 92
    Illegal BsaI.rc site found at 1782


Design Notes

the enzyme psr is used to both cut J70423 to insert the rbs mbp part and to cut the amplified rbs mbp part


Source

Maltose binding protein was amplified from the NEB vector pMAL-p5x using the primers 


RBS-MBP-GS-GFP start f

5'- GAA TTC GCG GCC GCT TCT AGA GAC GAA CGC TCT CTA CTA GAT GCC TAG AGT CG C CCC CTA AGG GCG GAG GTA GGA GAA ACT CAA ATG AAA ATC GAA GAA GGT AAA CTG GTA ATC TG -3'

RBS-MBP-GS-GFP start r

5'- CTG CAG CGG CCG CTA CTA GTA GTA ATA TAT GTT CGA TAG ATT TTA CGA GAA CC A GTC TGC GCG TCT TTC AGG -3'


References